Journal: Biomedicines

Article Title: Novel Medicine for Endometriosis and Its Therapeutic Effect in a Mouse Model

doi: 10.3390/biomedicines8120619

Figure Lengend Snippet: Light microscopy images of immunohistochemical analysis with anti-ER (estrogen receptor)-α, ER-β, and NGF (nerve growth factor) antibodies. (Magnification, 1000×). ( a ) Uterus, ( b ) peritoneum, and ( c ) intestine tissue. The results presented are the DAB-positive signal (brown) and hematoxylin (blue) for nuclear counterstaining; scale bar, 100 μm.

Article Snippet: Subsequently, the slides were stained with a mouse anti-estrogen receptor (ER)-α antibody (SC-8002, monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-ER-β antibody (SC-390243, monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA), and mouse monoclonal anti-nerve growth factor (NGF) antibody (11050-RP02, Sino Biological, Beijing, China) in a humidified chamber overnight at 4 °C.

Techniques: Light Microscopy, Immunohistochemical staining

Journal: Biomedicines

Article Title: Novel Medicine for Endometriosis and Its Therapeutic Effect in a Mouse Model

doi: 10.3390/biomedicines8120619

Figure Lengend Snippet: Comparative analysis of protein expression in the inflammatory lesions of endometriosis tissue using anti-ER-α, ER-β, CD61, and NGF antibodies. ( a ) Western blot images of ER-α, ER-β, CD61, and NGF protein expression patterns in the control and experimental groups (Dienogest, Cabergoline, and combined treatment). ( b ) Graph of Western blot band intensity of the control and experimental groups according to medication. All experiments were repeated three times for statistical analysis (±SD, ** p < 0.01 and *** p < 0.001).

Article Snippet: Subsequently, the slides were stained with a mouse anti-estrogen receptor (ER)-α antibody (SC-8002, monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA), mouse anti-ER-β antibody (SC-390243, monoclonal, Santa Cruz Biotechnology, Dallas, TX, USA), and mouse monoclonal anti-nerve growth factor (NGF) antibody (11050-RP02, Sino Biological, Beijing, China) in a humidified chamber overnight at 4 °C.

Techniques: Expressing, Western Blot

Journal: JOR Spine

Article Title: Age‐related molecular changes in the lumbar dorsal root ganglia of mice: Signs of sensitization, and inflammatory response

doi: 10.1002/jsp2.1124

Figure Lengend Snippet: List of primary and secondary antibodies used for immunofluorescence analysis

Article Snippet: rabbit anti‐NGF , BosterBio , m00341 , 1.00 , 1:100 , same as above.

Techniques: Immunofluorescence, Concentration Assay

Journal: Brain Sciences

Article Title: Ouabain Counteracts Retinal Ganglion Cell Death Through Modulation of BDNF and IL-1 Signaling Pathways

doi: 10.3390/brainsci15020123

Figure Lengend Snippet: Primary and secondary antibodies for Western blotting.

Article Snippet: Rabbit anti-NGF , PeproTech (Cranbury, NJ, USA) , 1:750.

Techniques: Western Blot

Journal: Oncotarget

Article Title: Long-term neurocognitive dysfunction in offspring via NGF/ ERK/CREB signaling pathway caused by ketamine exposure during the second trimester of pregnancy in rats

doi: 10.18632/oncotarget.16042

Figure Lengend Snippet: Ketamine administered during the second trimester perturbed NGF and down-regulated ERK, p-ERK, MSK, CREB, p-CREB, SYP, SYN, and PSD95 in the hippocampi of offspring. On postnatal day 30, after behavioral tests, brains were harvested for western blot analyses to determine the expression of NGF, ERK, p-ERK, MSK, CREB, p-CREB, SYP, SYN, and PSD95. (A–I ) Above are representative immunoblots for the expression levels of the corresponding proteins, and below are the histograms of the quantification of the corresponding proteins normalized to GAPDH. Data are expressed as mean percentage ± SEM of control mean values. * p < 0.05.

Article Snippet: The separated proteins were transferred onto nitrocellulose (NC) membranes and incubated with rabbit anti- GAPDH, NGF, ERK, p-ERK, CREB, p-CREB, MSK1, SYP, SYN, and PSD95 (1:600, EnoGene, Nanjing, China) overnight at a temperature of 4 °C.

Techniques: Western Blot, Expressing, Control

Journal: Oncotarget

Article Title: Long-term neurocognitive dysfunction in offspring via NGF/ ERK/CREB signaling pathway caused by ketamine exposure during the second trimester of pregnancy in rats

doi: 10.18632/oncotarget.16042

Figure Lengend Snippet: Ketamine administered during the second trimester down-regulated ERK, p-ERK, MSK, CREB, p-CREB, SYP, SYN, and PSD95 in PC12 cells during in vitro experiments. SCH772984 is the inhibitor of the ERK protein. Through the contrast between S group and C group, a very good effect of the inhibitor is revealed. (A) Representative immunoblots for expression levels of the corresponding proteins. (B–I ) Histograms that quantify the corresponding proteins normalized to GAPDH, in which the data are expressed as mean percentages ± SEM of control mean values. * p < 0.05

Article Snippet: The separated proteins were transferred onto nitrocellulose (NC) membranes and incubated with rabbit anti- GAPDH, NGF, ERK, p-ERK, CREB, p-CREB, MSK1, SYP, SYN, and PSD95 (1:600, EnoGene, Nanjing, China) overnight at a temperature of 4 °C.

Techniques: In Vitro, Western Blot, Expressing, Control